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| Status |
Public on Oct 01, 2008 |
| Title |
Characterization of the SigB-PrfA regulatory network in Listeria monocytogenes |
| Platform organisms |
Listeria monocytogenes; Listeria innocua |
| Sample organism |
Listeria monocytogenes |
| Experiment type |
Expression profiling by array
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| Summary |
Listeria monocytogenes SigB and PrfA are pleiotropic regulators of stress response and virulence gene expression, which have been shown to co-regulate genes in L. monocytogenes. We performed whole genome transcriptional profiling in the presence of PrfA* and active SigB, to identify the overlaps between the PrfA virulence regulon and the SigB stress response regulon. In L. monocytogenes, the PrfA* allele contributes to the activation of virulence genes to a level comparable to that of intracellular growing L. monocytogenes. Our results showed that the core PrfA regulon consists of 12 genes previously described as PrfA regulated. Furthermore, we found that the role of SigB during virulence gene regulation changes, dependent on the presence or absence of PrfA*. In the absence of PrfA*, SigB activated the transcription of virulence genes such as inlA and inlB. In the presence of PrfA*, SigB negatively influenced the transcription of genes in the PrfA core regulon. The observed effect of SigB on the transcript level of PrfA regulated genes was shown to reduce the cytotoxic effect of the PrfA* allele in HepG-2 cells. Our results indicate that the SigB-PrfA regulatory network is important for the adjustment of virulence gene transcription to ensure L. monocytogenes success as an intracellular pathogen.
Keywords: comparison of gene expression of regulatory mutants
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| Overall design |
The experimental design included 4 mutant strains of L. monocytogenes 10403S (PrfA*, delta prfA, delta prfA delta sigB, and PrfA* delta sigB), of which cDNA generated from 4 biological replicates were hybridized in all possible pairwise comparisons. Data were analyzed using a one way ANOVA in R/MAANOVA to determine significant differences in gene expression among the different strains. A two way ANOVA implemented in R/MAANOVA determined significant differences in gene expression due to the presence or absence of SigB and PrfA.
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| Contributor(s) |
Ollinger J, Bergholz TM, Wiedmann M, Boor KJ |
| Citation(s) |
19255187 |
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| Submission date |
May 05, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Teresa M Bergholz |
| E-mail(s) |
[email protected]
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| Organization name |
Cornell University
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| Department |
Food Science
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| Lab |
Food Safety Lab
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| Street address |
405 Stocking Hall
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL5029 |
CUFSL Listeria 6K oligonuclotide array v1.0 |
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| Samples (24)
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| GSM286188 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA rep 1 |
| GSM286189 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA, rep2 |
| GSM286190 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA, rep3 |
| GSM286191 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA, rep4 |
| GSM286192 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs PrfA*_delta sigB, rep1 |
| GSM286193 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs PrfA*_delta sigB, rep2 |
| GSM286194 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs PrfA*_delta sigB, rep3 |
| GSM286195 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs PrfA*_delta sigB, rep4 |
| GSM286196 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA_delta sigB, rep1 |
| GSM286197 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA_delta sigB, rep2 |
| GSM286198 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA_delta sigB, rep3 |
| GSM286199 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA* vs delta prfA_delta sigB, rep4 |
| GSM286200 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA, rep1 |
| GSM286201 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA, rep2 |
| GSM286202 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA, rep3 |
| GSM286203 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA, rep4 |
| GSM286612 |
Listeria monocytogenes PrfA-SigB co-regulon, delta prfA vs delta prfA delta sigB, rep1 |
| GSM286614 |
Listeria monocytogenes PrfA-SigB co-regulon, delta prfA vs delta prfA delta sigB, rep2 |
| GSM286615 |
Listeria monocytogenes PrfA-SigB co-regulon, delta prfA vs delta prfA delta sigB, rep3 |
| GSM286616 |
Listeria monocytogenes PrfA-SigB co-regulon, delta prfA vs delta prfA delta sigB, rep4 |
| GSM286617 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA delta sigB, rep1 |
| GSM286618 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA delta sigB, rep2 |
| GSM286642 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA delta sigB, rep3 |
| GSM286643 |
Listeria monocytogenes PrfA-SigB co-regulon, PrfA*_delta sigB vs delta prfA delta sigB, rep4 |
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| Relations |
| BioProject |
PRJNA106563 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11347_RAW.tar |
13.2 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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