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| Status |
Public on May 05, 2018 |
| Title |
Drosophila CNS mitochondrial dysfunction with Ras/MAPK inhibition microarray |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Mitochondrial dysfunction causes biophysical, metabolic and signalling changes that alter homeostasis and reprogram cells. We used a Drosophila model in which TFAM is overexpressed in the nervous system with or without Ras/MAPK pathway inhibition, by knock-down of the ETS transcription factor pointed, to investigate the how mitochondrial dysfunction and Ras/MAPK signalling affect the transcriptome.
We used microarray analysis to investigate gene expression in cases of mitochondrial dysfunction in the CNS with or without Ras/MAPK pathway inhibition by knock-down of pointed (Pnt) and anterior open (Aop).
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| Overall design |
RNA was purified from the late third instar larval CNS from control larvae, or larvae over-expressing mitochondrial transcription factor A (TFAM) in post-mitotic neurons using the neuron specific driver nSyb-Gal4, or larvae over-expressing TFAM in combination with knock-down of Pnt or Aop. nSyb-Gal4 driving Pnt or Aop knock-down alone conditions were also included. Three replicates are included for all conditions.
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| Contributor(s) |
Bateman JM, Duncan OF |
| Citation(s) |
30059502 |
| |
| Submission date |
May 04, 2018 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Joseph M. Bateman |
| E-mail(s) |
[email protected]
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| Phone |
2078488144
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| Organization name |
King's College London
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| Street address |
Guy's campus
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| City |
London |
| State/province |
(Click to Select US State) |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA463815 |
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