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| Status |
Public on Sep 01, 2018 |
| Title |
Transcriptomic analysis of engineered and improved Pichia pastoris for S-adenosyl-l-methionine (SAM) production |
| Platform organism |
Saccharomyces cerevisiae |
| Sample organism |
Komagataella phaffii GS115 |
| Experiment type |
Expression profiling by array
|
| Summary |
SAM is the principal methyl group donor in all living organisms, and has attracted much interest in clinical research. We improved SAM production in engineered P. pastoris strain GS115/DS56 by overexpression of the recombinant methionine adenosyltransferase (MAT) gene DS56, and further enhanced SAM prduction in G12-CBS by downregulation of the cystathionine β-synthase (CBS) gene CYS4 with a weak promoter G12. We performed the pairwise transcriptome comparisons between high-producing (HP) and low-producing (LP) strains, in order to understand the impact of SAM accumulation on the P. pastoris physiology and to identify genome-wide targets for further improving SAM production.
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| Overall design |
A twelve array study using total RNA recovered from the LP control strain GS115/3.5K, and the HP strains GS115/DS56 and G12-CBS grown in shake flask at 72 h after methanol induction in the presence of L-Met, as well as from the strain G12-CBS just before L-Met feeding after methanol induction for 24 h. Each chip measures the expression levels of 5777 genes (8 probes per gene).
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| Contributor(s) |
Qian J, Qin X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 08, 2018 |
| Last update date |
Sep 02, 2018 |
| Contact name |
Xiulin Qin |
| E-mail(s) |
[email protected]
|
| Organization name |
Guangxi university
|
| Department |
College of Life Science and Technology
|
| Street address |
100 Daxue Road
|
| City |
Nanning |
| State/province |
Guangxi |
| ZIP/Postal code |
530004 |
| Country |
China |
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| Platforms (1) |
| GPL17988 |
NimbleGen Saccharomyces cerevisiae 12x135K Array [10078_Scer_EXP_HX12] |
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| Samples (12)
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| GSM3135690 |
GS115/3.5K_LP strain induced with methanol for 72 h_rep1 |
| GSM3135691 |
GS115/3.5K_LP strain induced with methanol for 72 h_rep2 |
| GSM3135692 |
GS115/3.5K_LP strain induced with methanol for 72 h_rep3 |
| GSM3135693 |
GS115/DS56_HP strain induced with methanol for 72 h_rep1 |
| GSM3135694 |
GS115/DS56_HP strain induced with methanol for 72 h_rep2 |
| GSM3135695 |
GS115/DS56_HP strain induced with methanol for 72 h_rep3 |
| GSM3135696 |
G12-CBS_HP strain induced with methanol for 72 h_rep1 |
| GSM3135697 |
G12-CBS_HP strain induced with methanol for 72 h_rep2 |
| GSM3135698 |
G12-CBS_HP strain induced with methanol for 72 h_rep3 |
| GSM3135699 |
G12-CBS_HP strain induced with methanol for 24 h_rep1 |
| GSM3135700 |
G12-CBS_HP strain induced with methanol for 24 h_rep2 |
| GSM3135701 |
G12-CBS_HP strain induced with methanol for 24 h_rep3 |
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| Relations |
| BioProject |
PRJNA464437 |
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