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| Status |
Public on Sep 13, 2018 |
| Title |
Microarray based gene expression profiling of RNA binding protein, RBM10 in HEK 293 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine the target genes of RBM10,we have employed microarray based gene expression profiling by knocking down RBM10 in HEK 293 cells. Microarray analysis after RBM10 knockdown on HEK 293 cells showed that over 1000 genes were down regulated while another 800 genes up regulated as a result of the knockdown. Among the down regulated genes, we found the significant presence of cardiovascular disease related genes, especially cardiac hypertrophy and heart failure.
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| Overall design |
HEK 293 cells was transfected with siRNA oligos (both control siRNA and RBM10 specific siRNA) using Oligofectamine reagent and cells were harvested 48-hours post-transfection. Three independent experiments were conducted for both control siRNA and RBM10 siRNA treatment.
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| Web link |
https://www.cell.com/cell-reports/fulltext/S2211-1247(18)31381-0
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| |
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| Contributor(s) |
Mohan N, Laishram RS |
| Citation(s) |
30257214 |
| |
| Submission date |
May 14, 2018 |
| Last update date |
Dec 14, 2018 |
| Contact name |
Rakesh S Laishram |
| E-mail(s) |
[email protected]
|
| Organization name |
Rajiv Gandhi Centre for Biotechnology(RGCB)
|
| Department |
Cardiovascular and Diabetes Biology Group
|
| Lab |
Cardiovascular and Diabetes Biology Lab
|
| Street address |
Thycaud P O, Poojappura
|
| City |
Trivandrum |
| State/province |
Kerala |
| ZIP/Postal code |
695014 |
| Country |
India |
| |
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| Platforms (1) |
| GPL21061 |
Agilent-039494 8x60k Human Whole Genome Microarray Gene expression (Probe name version) |
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| Samples (6)
|
| GSM3141705 |
total RNA from control siRNA treated sample 1 |
| GSM3141706 |
total RNA from control siRNA treated sample 2 |
| GSM3141707 |
total RNA from control siRNA treated sample 3 |
| GSM3141708 |
total RNA from RBM10 siRNA treated sample 1 |
| GSM3141709 |
total RNA from RBM10 siRNA treated sample 2 |
| GSM3141710 |
total RNA from RBM10 siRNA treated sample 3 |
|
| Relations |
| BioProject |
PRJNA471343 |
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