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| Status |
Public on Jan 21, 2019 |
| Title |
SpyCLIP: An easy-to-use and high-throughput compatible platform for efficient characterization of protein-RNA interactions |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We have developed a covalent linkage-based SpyCLIP method by taking advantage of SpyTag-SpyCatcher chemistry, which circumvents gel purification steps of captured RBP-RNA needed for all existing CLIP methods, thereby dramatically reducing the technical difficulty and enabling high-throughput application of the CLIP technology.
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| Overall design |
SpyCLIP and corresponding input sample for PTBP1, SLBP, RBFOX2 and AGO2 in Lenti-X 293T cell line.
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| |
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| Contributor(s) |
Zhao Y, Zhang Y, Teng Y, Liu K, Liu Y, Li W, Wu L |
| Citation(s) |
30715466 |
| |
| Submission date |
May 21, 2018 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Ligang Wu |
| E-mail(s) |
[email protected]
|
| Phone |
+86-21-54921321
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institutes for biological Sciences
|
| Lab |
Wu ligang
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA472368 |
| SRA |
SRP148580 |
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