 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2020 |
| Title |
MLL RIP-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We uncovered an unexpected function of mixed-lineage leukemia (MLL) protein, which is a critical epigenetic factor and whose disruption leads to leukemogenesis, in the functional regulation of miRNAs. We reveal that the MLLC180 subunit alone can colocalize with miRNA-induced silencing complex (miRISC) components in cytoplasm processing bodies (P-bodies), where miRNA-mediated gene silencing takes place. In addition, we show that MLL was specifically required for miRNA-mediated translational repression, but not for miRNA or siRNA-mediated mRNA cleavage. Mechanistically, MLL was necessarily required for recruiting miRNA to the miRISC and P-body core, partly through its binding partner RAN. Furthermore, dysregulation of the miRNA repression function in MLL leukemic cells was essential for high-level expression of MYC, which ensured the survival of MLL leukemic cells. Thus, our investigation discovered that the MLL subunit alone can exert other functions in miRNA-mediated gene silencing apart from the epigenetic function of the whole MLL heterodimer. We also demonstrated as proof-of-principle that functional deregulation of miRNA may play an important role in cancer.
|
| |
|
| Overall design |
Identify the binding RNAs and small RNAs of the MLL protein using RIP-seq method
|
| |
|
| Contributor(s) |
Zhu S, Liu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 15, 2018 |
| Last update date |
Jan 02, 2020 |
| Contact name |
rui heng wang |
| E-mail(s) |
[email protected]
|
| Phone |
1858057173
|
| Organization name |
shanghaijiaotong university
|
| Department |
insitute of hematology
|
| Lab |
lh708
|
| Street address |
ruijin two street
|
| City |
shanghai |
| State/province |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA476300 |
| SRA |
SRP150613 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE115848_RAW.tar |
3.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
