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| Status |
Public on Jun 27, 2018 |
| Title |
E. coli transcriptome-level alterations upon antimicrobial peptide exposure |
| Platform organisms |
Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
E. coli (P1645ec: a clinical isolate) was incubated with differing concentrations of antimicrobial peptides (all at 0.5X MBC)
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| Overall design |
E. coli (P1645ec: a clinical isolate) was incubated with differing concentrations of antimicrobial peptides omega76, omega17, and NN2_0018 (all at 0.5X MBC). 3 treated samples (2 biological replicates each) and 1 untreated samples (2 biological replicates) were used in this study. All cultures were grown in 10 ml LB broth at 37C/180rpm for 24 hours before flash-freezing & RNA extraction
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| |
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| Contributor(s) |
Nagarajan D, Ravichandran S, Chandra N |
| Citation(s) |
29259134 |
| |
| Submission date |
Jun 26, 2018 |
| Last update date |
Jun 30, 2018 |
| Contact name |
Deepesh Nagarajan |
| E-mail(s) |
[email protected]
|
| Phone |
+91 9892612638
|
| Organization name |
Indian Institute of Science
|
| Department |
Department of Biochemistry
|
| Lab |
Prof. Nagasuma Chandra Lab
|
| Street address |
FB-04, B-wing, Department of Biochemistry, New Biology Building, IISc
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
| |
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| Platforms (1) |
| GPL13359 |
Agilent-020097 E. coli Gene Expression Microarray (Probe Name version) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA478027 |
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