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| Status |
Public on Mar 01, 2019 |
| Title |
RNA-seq data showing the effects of rpoD mutation t1682c in Pseudomonas fluorescens |
| Organism |
Pseudomonas [fluorescens] SBW25 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report RNA-seq data for four Pseudomonas fluorescens SBW25 types: SBW25 (wild type) and dervied types 6A4, 6B4-Cap- and 6B4-Cap+. The aim of the study is to see the effect of a mutation in rpoD (carried by 6B4) on the transcriptome.
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| Overall design |
Method: 3x single colonies of each of SBW25, 6A4 and 6B4 were grown on KB agar, precultured in shaken KB, grown to mid-exponential phase in shaken KB flasks. Total mRNA isolated with RiboPureTM Bacteria Kit (Ambion®). Note that 6B4 was separated into Cap- and Cap+ fractions by centrifugation before RNA extraction. Normalized mRNA-seq library preparation, followed by 100 bp single end sequencing, was performed by the Australian Genome Research Facility (Brisbane, Australia).
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| Contributor(s) |
Gallie J, Bertels F, Ferguson GC, Rainey PB |
| Citation(s) |
30835268 |
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| Submission date |
Jul 02, 2018 |
| Last update date |
Jun 02, 2019 |
| Contact name |
Jenna Gallie |
| E-mail(s) |
[email protected]
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| Organization name |
Max Planck Institute for Evolutionary Biology
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| Street address |
August-Thienemann Strasse 2
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| City |
Ploen |
| ZIP/Postal code |
24306 |
| Country |
Germany |
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| Platforms (1) |
| GPL25322 |
Illumina HiSeq 2500 (Pseudomonas fluorescens SBW25) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA478834 |
| SRA |
SRP151740 |
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