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| Status |
Public on Jul 07, 2018 |
| Title |
Light enhances growth in non-phototropic Actinobacteria (Aurantimicrobium sp. MWH-Mo1) |
| Organism |
Aurantimicrobium photophilum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Light is a source of energy and an environmental cue that is available in excess in most surface environments. In prokaryotic systems, conversion of light to energy by photoautotrophs and photoheterotrophs is well understood, but the conversion of light to information and the cellular response to that information has been characterized in only a few species. Our goal was to explore the response of freshwater Actinobacteria, which are ubiquitous in illuminated aquatic environments, to light. We found that Actinobacteria without functional photosystems grow faster in the light, likely because sugar transport and metabolism are upregulated in the light, while protein synthesis is upregulated in the dark. Based on the action spectrum of the growth effect, and comparisons of the genomes of three Actinobacteria with this growth rate phenotype, we propose that the photosensor in these strains is a putative CryB-type cryptochrome. The ability to sense light and upregulate carbohydrate transport during the day could allow these cells to coordinate their time of maximum organic carbon uptake with the time of maximum organic carbon release by primary producers.
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| Overall design |
Two bacterial strains (Rhodoluna lacicola and Aurantimicrobium sp. strain MWH-Mo1) grown in constant light or constant darkness until late exponential phase; 4 replicates each
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| Contributor(s) |
Keffer JL, Hempel P, Polson SW, Maresca JA |
| Citation missing |
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| Submission date |
Jul 06, 2018 |
| Last update date |
Jul 08, 2018 |
| Contact name |
Shawn William Polson |
| E-mail(s) |
[email protected]
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| Organization name |
University of Delaware
|
| Department |
Center for Bioinformatics and Computational Biology
|
| Lab |
AP Biopharmaceutical Innovation Center
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| Street address |
590 Avenue 1743
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| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19713 |
| Country |
USA |
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| Platforms (1) |
| GPL25296 |
Illumina HiSeq 2500 (Aurantimicrobium sp. MWH-Mo1) |
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| Samples (8)
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| GSM3258860 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant light. Replicate 1 |
| GSM3258861 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant light. Replicate 2 |
| GSM3258862 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant light. Replicate 3 |
| GSM3258863 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant light. Replicate 4 |
| GSM3258864 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant dark. Replicate 1 |
| GSM3258865 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant dark. Replicate 2 |
| GSM3258866 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant dark. Replicate 3 |
| GSM3258867 |
Aurantimicrobium sp. strain MWH-Mo1, grown in constant dark. Replicate 4 |
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| Relations |
| BioProject |
PRJNA479963 |
| SRA |
SRP152496 |
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