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| Status |
Public on Jul 30, 2018 |
| Title |
Whole head tissue of mated female w1118 Drosophila melanogaster exposed to 20% w/v coconut oil in solid medium high fat diet |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Obesity predisposes humans and other mammals to a range of life-threatening comorbidities, including type 2 diabetes and cardiovascular disease. Obesity and its associated dietary habits also aggravate neural pathologies, such as Alzheimer’s disease, but this class of comorbidity is less understood. When Drosophila melanogaster (flies) are exposed to high fat diet (HFD) by supplementing a standard cornmeal-sucrose-yeast medium with coconut oil, they adopt an obese phenotype of decreased lifespan, increased triglyceride storage, and hindered climbing ability. The latter development is an indicator of neurological decline in flies. Our objective was to establish the obesity-like phenotype in Drosophila and identify a correlation, if any, between obesity and neurological decline in flies through behavioral and expression microarray. We found that mated female w1118 flies exposed to HFD maintained an obese phenotype throughout adult life with onset at seven days, evidenced by increased triglyceride stores, diminished life span, and impeded climbing ability. Analysis of gene expression of the fly head via microarray and qRT-PCR validation revealed functionally relevant genes with significant fold changes. These genes had functions including in memory, metabolism, olfaction, mitosis, cell signaling, and motor function. An Aversive Phototaxis Suppression assay indicated short term memory impairment as a result of HFD. Meanwhile, there was a decline but no significant difference in odor-seeking ability with HFD. Overall, our results point to the suitability of Drosophila melanogaster to investigate connections between diet-induced obesity and nervous or neurobehavioral pathology, and to the existence of such a dynamic in an evolutionarily broad range of organisms.
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| Overall design |
3 biological replicates of mated females of genotype w1118 exposed to a high fat diet vs. 3 biological replicates of mated females of genotype w1118 exposed to a normal diet (which lacks the source of dietary fat). Mated females were synchronously mated with isogenic males of the same background across diet group. Mated females were exposed to their respective diets for 7 days. Heads were removed by snap freezing and vibration. Total RNA was extracted. Gene expression levels in the high fat diet relative to the normal diet were obtained by Affymetrix 2.0 Genome Arrays and compared.
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| Contributor(s) |
Talbert ME, Rivera O |
| Citation(s) |
30810797 |
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| Submission date |
Jul 29, 2018 |
| Last update date |
Aug 26, 2019 |
| Contact name |
Matthew Edward Talbert |
| E-mail(s) |
[email protected]
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| Organization name |
University of Louisiana at Monroe
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| Department |
Biology
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| Street address |
700 University Avenue
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| City |
Monroe |
| State/province |
LA |
| ZIP/Postal code |
71209 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA483349 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE117834_RAW.tar |
12.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
| Processed data included within Sample table |
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