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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 24, 2020 |
| Title |
Expression data of ex vivo purified CD4+ cells from WT and P2rx7-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Extracellular adenosine triphosphate (eATP) is a signaling molecule that affects T cell function via the ionotropic P2X7 receptor. The study of effector/memory T cells isolated from mice with deletion of P2rx7, the gene encoding for P2X7, allowed understanding the impact of P2X7 activity on T cell function in the eATP-rich tumor microenvironment.
To explore the the transcriptional impact of the lack of P2rx7 in CD4+ naïve and TEM cells, we performed genome-wide expression profiling of ex vivo purified CD4+ naïve and TEM cells from WT and P2rx7-/- mice
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| Overall design |
CD4+ naïve and TEM cells were isolated from secondary lymphoid organs of WT and P2rx7-/- mice. Gene expression profiling was performed to identify transcripts modulated by eATP via P2X7 that coud affect the anti-tumor adaptive immune response.
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| Contributor(s) |
Bicciato S, Grassi F, Romagnani A, Rottoli E |
| Citation(s) |
32699136 |
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| Submission date |
Aug 06, 2018 |
| Last update date |
Jul 26, 2020 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
[email protected]
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL25426 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate [CDF: HTMG430PM_Mm_ENTREZG_22.0.0] |
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| Samples (10)
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| GSM3319510 |
CD4+ naïve cells from WT mice, biological rep1 |
| GSM3319511 |
CD4+ naïve cells from WT mice, biological rep2 |
| GSM3319512 |
CD4+ naïve cells from P2rx7-/- mice, biological rep1 |
| GSM3319513 |
CD4+ naïve cells from P2rx7-/- mice, biological rep2 |
| GSM3319514 |
CD4+ TEM cells from WT mice, biological rep1 |
| GSM3319515 |
CD4+ TEM cells from WT mice, biological rep2 |
| GSM3319516 |
CD4+ TEM cells from WT mice, biological rep3 |
| GSM3319517 |
CD4+ TEM cells from P2rx7-/- mice, biological rep1 |
| GSM3319518 |
CD4+ TEM cells from P2rx7-/- mice, biological rep2 |
| GSM3319519 |
CD4+ TEM cells from P2rx7-/- mice, biological rep3 |
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| Relations |
| BioProject |
PRJNA484706 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118146_RAW.tar |
31.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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