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| Status |
Public on Dec 07, 2018 |
| Title |
Transcriptional profile of Mycobacterium tuberculosis espL deletion mutant |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis. The ESX-1 cluster comprises approximately twenty genes and encodes a specialized secretion apparatus, which releases effectors into the extracellular milieu. The goal of this study is to understand the role of EspL. We show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate innate cytokine production. Moreover, loss of EspL also leads to downregulation in M. tuberculosis of WhiB6, a redox-sensitive transcriptional activator of ESX-1 genes.
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| Overall design |
delta espL vs. wild-type
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| Contributor(s) |
Benjak A, Sala C, Cole ST |
| Citation(s) |
30571761 |
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| Submission date |
Aug 23, 2018 |
| Last update date |
Feb 20, 2019 |
| Contact name |
Andrej Benjak |
| E-mail(s) |
[email protected]
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| Organization name |
University of Bern
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| Department |
Department for BioMedical Research
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| Street address |
Murtenstrasse 24-28
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| City |
Bern |
| ZIP/Postal code |
3008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17879 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis) |
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| Samples (4)
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| GSM3356372 |
M. tuberculosis H37Rv, delta espL replicate 2 |
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| Relations |
| BioProject |
PRJNA487629 |
| SRA |
SRP158711 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118994_CountTable_no-rRNA.txt.gz |
42.1 Kb |
(ftp)(http) |
TXT |
| GSE118994_DESeq2_delta-espL_vs_wt.csv.gz |
187.3 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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