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| Status |
Public on Oct 23, 2018 |
| Title |
Array CGH data of A. oryzae strains bearing translocated chromosomal duplication and triplication |
| Platform organism |
Aspergillus oryzae RIB40 |
| Sample organism |
Aspergillus oryzae |
| Experiment type |
Genome variation profiling by array
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| Summary |
In this study, we achieved translocated chromosomal duplication and triplication of a 1.4-Mb targeted chromosomal region by directly introducing I-SceI meganuclease into A. oryzae protoplast cells. Strains with duplication and triplication of chromosome 2 showed substantial increases in the activity of protease and amylase. Gene dosage effects were enhanced by combining the structural gene and its regulatory gene, indicating that segmental duplications of chromosomes play important phenotypic roles in koji mold strains.
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| Overall design |
A. oryzae strain bearing a 1.4Mb translocated chromosomal duplication, A. oryzae strain bearing a 1.4Mb translocated chromosomal triplication, and A. oryzae RIB40 (wild type strain), were cultivated in Polypeptone-dextrin medium. After 3 days cultivation, genomic DNAs from the samples were extracted, and array CGH analysis was carried out to confirm the chromosomal duplications in the strains.
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| Contributor(s) |
Takahashi T, Ogawa M, Sato A, Koyama Y |
| Citation(s) |
30305913 |
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| Submission date |
Sep 28, 2018 |
| Last update date |
Oct 23, 2018 |
| Contact name |
Tadashi Takahashi |
| E-mail(s) |
[email protected]
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| Organization name |
Noda Institute for Scientific Research
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| Street address |
399 Noda
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| City |
Noda City |
| ZIP/Postal code |
278-0037 |
| Country |
Japan |
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| Platforms (1) |
| GPL16017 |
Agilent_017367 NISR Aspergillus oryzae 24K v.2.0 |
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| Samples (2) |
| GSM3405623 |
aCGH data of J4: Aspergillus oryzae strain bearing a 1.4Mb translocated chromosomal duplication |
| GSM3405624 |
aCGH data of I-8: Aspergillus oryzae strain bearing a 1.4Mb translocated chromosomal triplication |
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| Relations |
| BioProject |
PRJNA493741 |
