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| Status |
Public on Apr 25, 2019 |
| Title |
Age-dependent changes in transcription factor FOXO targeting in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
FOXO transcription factors control numerous pathways involving metabolism, stress response, and longevity. Although direct targets of FOXO have been reported in various long-lived mutants and under stress conditions, no studies have investigated how normal aging impacts the cellular activity of FOXO. In this study, we compared genome wide dFOXO-bound sites in young and aged wild-type flies kept under normal feeding conditions to evaluate the dynamics of FOXO gene targeting during aging. Our results provide a new insight into FOXO chromatin targeting under a normal aging model and highlight the diverse regulatory mechanisms for FOXO transcriptional activity that are currently less understood.
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| Overall design |
ChIP DNA was prepared from 2-week and 5-week yw;+;+ flies. Two biological replicates were collected for each sample using dFOXO antibody. ChIP-seq libraries were prepared using the standard protocol with indexed adaptors and pooled for sequencing.
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| Contributor(s) |
Birnbaum A, Bai H |
| Citation(s) |
31134124 |
| |
| Submission date |
Oct 10, 2018 |
| Last update date |
Apr 22, 2021 |
| Contact name |
Allison Birnbaum |
| E-mail(s) |
[email protected], [email protected]
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| Organization name |
Iowa State University
|
| Street address |
3247 Pammel Dr
|
| City |
Ames |
| State/province |
AL |
| ZIP/Postal code |
50010 |
| Country |
USA |
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| Platforms (1) |
| GPL23323 |
Illumina HiSeq 3000 (Drosophila melanogaster) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA495643 |
| SRA |
SRP164947 |
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