|
| Status |
Public on Jul 10, 2009 |
| Title |
FACS-Assisted Microarray Profiling Implicates Novel Genes and Pathways in Zebrafish Gastrointestinal Tract Development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Zebrafish (Danio rerio) gutGFP transgenic embryos [Tg(XlEef1a1:GFP)s854] were collected at 4 time points: 2 days post fertilization (dpf), 3, dpf, 4 dpf, 6 dpf. Embryos were dissociated into single cells and sorted by FACS based on GFP expression.
RNA was extracted from the different cell populations (Stratagene), amplified (NuGEN Ovation), and hybridized to Affymetrix Zebrafish GeneChips.
Keywords: Time course.
|
| |
|
| Overall design |
Series of 24 samples: 4 time points, 3 replicates, 2 samples per replicate (GFP+ and GFP-).
|
| |
|
| Contributor(s) |
Stuckenholz C, Lu L, Thakur P, Kaminski N, Bahary N |
| Citation(s) |
19563808 |
| |
| Submission date |
Jul 21, 2008 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Carsten Stuckenholz |
| E-mail(s) |
[email protected]
|
| Phone |
(412) 648-3159
|
| Fax |
(412) 383-5918
|
| Organization name |
University of Pittsburgh
|
| Department |
Department of Medicine, Division of Hematology and Oncology
|
| Lab |
Bahary
|
| Street address |
3501 Fifth Avenue, Room 5065.5B
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15260 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA113405 |
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