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| Status |
Public on Mar 06, 2019 |
| Title |
iPSC-Derived Brain Endothelium Exhibits Stable, Long-Term Barrier Function in Perfused Hydrogel Scaffolds |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We describe the construction a 3D blood-brain barrier model comprised of iPSC-derived brain endothelium cultured in channels within gelatin hydrogels. The iPSC-derived brain endothelium displays key features of the blood-brain barrier phenotype, including tight junction formation, efflux transporter activity, low paracellular permeability, and suppressed levels of transcytosis compared to non-blood-brain barrier endothelial controls. Collectively, this work provides the foundation for a fully human, biomimetic neurovascular model to study BBB in health and disease.
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| Overall design |
iPSC-derived brain endothelium was cultured in 3D channels in gelatin hydrogels and analyzed for key signatures of blood-brain barrier phenotype. RNA-seq was used to assess changes in global gene expression signatures between endothelium cultured for one day under static conditions and fourteen days under perfused conditions.
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| Contributor(s) |
Neal EH, Faley SL, Bellan LM, Lippmann ES |
| Citation(s) |
30773484 |
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| Submission date |
Nov 15, 2018 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Ethan Lippmann |
| E-mail(s) |
[email protected]
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| Organization name |
Vanderbilt University
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| Department |
Chemical and Biomolecular Engineering
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| Lab |
Lippmann Lab
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| Street address |
2400 Highland Ave, Room 303
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37212 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA505675 |
| SRA |
SRP168818 |
