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| Status |
Public on Nov 16, 2018 |
| Title |
Dynamic expression of Id3 defines the stepwise differentiation of tissue-resident regulatory T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Foxp3+ regulatory T (TR) cells are phenotypically and functionally diverse, and broadly distributed in lymphoid and non-lymphoid tissues. However, the pathways guiding the differentiation of tissue-resident TR populations have not been well defined. By regulating E-protein function, Id3 controls the differentiation of CD8+ effector T cells and is essential for TR maintenance and function. We show that dynamic expression of Id3 helps define three distinct mouse TR populations, Id3+CD62LhiCD44lo central (c)TR, Id3+CD62LloCD44hi effector (e)TR and Id3- eTR. Adoptive transfer experiments and transcriptome analyses support a stepwise model of differentiation from Id3+ cTR to Id3+ eTR to Id3- eTR. Furthermore, Id3- eTR have high expression of functional inhibitory markers and a transcriptional signature of tissue-resident TR. Accordingly, Id3- eTR are highly enriched in non-lymphoid organs, but virtually absent from blood and lymph. Thus, we propose that tissue-resident TR develop in a multi-step process associated with Id3 downregulation.
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| Overall design |
Biological triplicates from three different populations in lymph node and spleen.
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| Contributor(s) |
Sullivan JM, Höllbacher B, Campbell DJ |
| Citation(s) |
30518568 |
| |
| Submission date |
Nov 15, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Daniel J Campbell |
| Organization name |
Benaroya Research Institute
|
| Street address |
1201 Ninth Avenue
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98101 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA505690 |
| SRA |
SRP168931 |
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