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| Status |
Public on Nov 14, 2019 |
| Title |
Embryonic response to high beta-hydroxybutyrate (BHB) levels in postpartum dairy cows [methylation] |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by array
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| Summary |
This project is meant to document the effect of the negative energetic balance on the embryo quality and as it happens, to identify ways to improve the fertility of dairy bovines. Measurement of the β-Hydroxybutyric acid (BHB) has been taken between 45 and 60 days post-partum on twelve Holstein cows. Cows have been ranked according to the sanguine measurement as high or low in BHB in order to get 6 cows per group. After having synchronized the heat with hormones and an ovarian stimulation, cows have been inseminated and the embryos were selected as so to be transferred in at least two heifers. With this transfer it is possible to isolate the impact of the energetic level of the mother before and during the gestation on the performances of the future progeny.
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| Overall design |
This is a 2 conditions experiment with morula (4 per pool) and blastocysts (1 per pool) collected in cows with low or high BHB. Four biological replicates for each treatment and each were hybridized in a dye swap design.
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| Contributor(s) |
Chaput C, Sirard M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 16, 2018 |
| Last update date |
Nov 15, 2019 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
[email protected]
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| Organization name |
Université Laval
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| Department |
Sciences Animales
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| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
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| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL18384 |
EmbryoGENE DNA Methylation Array Bovine 2x400K v2.0 |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE122646 |
Embryonic response to high beta-hydroxybutyrate (BHB) levels in postpartum dairy cows |
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| Relations |
| BioProject |
PRJNA505840 |
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