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| Status |
Public on Nov 30, 2018 |
| Title |
microRNA-seq reveals microRNAs under the regulation of miR-125b in NCCIT tumor cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
To investigate the role and mechanism of miR-125b on NCCIT cells without bias, we analyzed differentially expression microRNA profile among miR-125b antagomir-, miR-125b agomir-, and negative control-transfected NCCIT tumor cells by microRNA-seq.
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| Overall design |
Total RNA (1 μg for each samples) was extracted from miR-125b antagomir-, miR-125b agomir-, and control oligonucleotide (GenePharma, Shanghai, China)-transfected NCCIT (ATCC® CRL-2073) cells using miRcute miRNA Isolation Kit (Tiangen, Beijing, China). Three duplicate samples for each treatment were included. Then, equal amounts of total RNA were analysed using BGISEQ-500 platform and the sequencing procedure was performed by BGI (Shenzhen, China)
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| Contributor(s) |
Batool A, Chen S |
| Citation(s) |
30237497 |
| |
| Submission date |
Nov 30, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Su-Ren Chen |
| E-mail(s) |
[email protected]
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| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Stem Cell and Reproductive Biology
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| Street address |
1Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE123157 |
RNA-seq and microRNA-seq reveal RNAs and microRNAs under the regulation of miR-125b in NCCIT tumor cells |
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| Relations |
| BioProject |
PRJNA507722 |
| SRA |
SRP171577 |
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