|
| Status |
Public on Dec 12, 2008 |
| Title |
Comparison between Saccharomyces cerevisiae HS 10, HS 34 and HS 12, HS 37 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed.
The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then the Qiagen RNeasy Midi Kit with some modifications within the manufacturers´ protocol and the Qiagen RNase-Free DNase Set were applied.
6 µg of the total RNA per sample was used for each microarray experiments. The indirect labelling by the tyramide-signal-amplification method (MicromaxTM TSATM labelling and detection Kit from Perkin Elmer life sciences) was used to increase the Cy3 and Cy5 signals of microarray detection.
Each cDNA containing Biotin- and Fluorescein-nucleotides respectively was purified with a QIAquick PCR purification kit and suspended in 11 µl of the formamide containing hybridization buffer. The slides were hybridized at 42°C over night under a cover slip.
The microarrays were scanned by the Axon 4000B scanner; image intensity data were extracted and analysed with GenePix® Pro 6.0 software.
Data from different scans of Dye-swap experiment were extracted by GenePix Pro 6.0 software, normalized and united. An outliertest has been applied in order to find outliers amongst the gene replicats. Subsequently, a t-test (1% and 5% probability of error) has been used in order to find regulated genes.
Keywords: sorted yeast cells
|
| |
|
| Overall design |
Microarray experiments were realised with dye swap.
|
| |
|
| Contributor(s) |
Heine F, Stahl F, Sträuber H, Wiacek C, Benndorf D, Repenning C, Schmidt F, Scheper T, von Bergen M, Harms H, Müller S |
| Citation(s) |
19072835 |
| |
| Submission date |
Aug 07, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Franziska Heine |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz-Centre for Environmental Research - UFZ
|
| Department |
Environmental Microbiology
|
| Lab |
Flow Cytometry
|
| Street address |
Permoserstraße 15
|
| City |
Leipzig |
| ZIP/Postal code |
04315 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL7145 |
IFTC Saccharomyces cerevisiae 154 v1.0 |
|
| Samples (10)
|
| GSM310494 |
Flocculent strain HS 10 exponential growth phase vs powdery strain HS 37 exponential growth phase |
| GSM310495 |
Flocculent strain HS 10 exponential growth phase vs powdery strain HS 37 exponential growth phase (Dye swap) |
| GSM310496 |
Flocculent strain HS 10 exponential growth phase vs powdery strain HS 12 exponential growth phase |
| GSM310497 |
Flocculent strain HS 10 exponential growth phase vs powdery strain HS 12 exponential growth phase (Dye swap) |
| GSM310499 |
Flocculent strain HS 34 exponential growth phase vs powdery strain HS 37 exponential growth phase |
| GSM310500 |
Flocculent strain HS 34 exponential growth phase vs powdery strain HS 37 exponential growth phase (Dye swap) |
| GSM310501 |
Flocculent strain HS 34 exponential growth phase vs powdery strain HS 12 exponential growth phase |
| GSM310502 |
Flocculent strain HS 34 exponential growth phase vs powdery strain HS 12 exponential growth phase (Dye swap) |
| GSM310503 |
Flocculent strain HS 10 stationary growth phase vs powdery strain HS 37 stationary growth phase |
| GSM310504 |
Flocculent strain HS 10 stationary growth phase vs powdery strain HS 37 stationary growth phase (Dye swap) |
|
| Relations |
| BioProject |
PRJNA113201 |
Less...
More...