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| Status |
Public on Dec 31, 2018 |
| Title |
Mus musculus intestinal epithelial MODE-K cells incubated with Heligmosomoides bakeri secreted extracellular vesicles (EVs) |
| Organisms |
Mus musculus; Heligmosomoides polygyrus bakeri |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Small RNAs are emerging as important molecules for cross-species communication. Thanks to available and affordable sequencing technologies it is now possible to sequence small RNAs (sRNA-Seq) present in samples of interacting organisms. A first step when analyzing sRNA-Seq of two interacting species is to determine which sequences are being produced by which organism. Due to their small size (18-30), small RNAs could easily map to both host and parasite genomes. Here we produced data for Mus musculus intestinal epithelial cells treated with Extracellular Vesicles (EV) produced by the parasitic nematode Heligmosomoides bakeri.
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| Overall design |
MODE-K cells were incubated with H. bakeri vesicles for 4 and 24 hours, cells were washed with PBS buffer before small RNA sequencing, three biological replicates were generated for each condition. Sequencing was performed on an Illumina HiSeq high output v4
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| Contributor(s) |
Bermudez-Barrientos JR, Ramirez-Sanchez O, Chow FW, Buck AH, Abreu-Goodger C |
| Citation(s) |
31879784 |
| |
| Submission date |
Dec 30, 2018 |
| Last update date |
Mar 09, 2020 |
| Contact name |
José Roberto Bermúdez-Barrientos |
| E-mail(s) |
[email protected], [email protected], [email protected]
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| Organization name |
The University of Edinburgh
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| Department |
Institute of Immunology & Infection
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| Lab |
Buck Lab
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| Street address |
Charlotte Auerbach Rd
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| City |
Edinburgh |
| State/province |
Lothian |
| ZIP/Postal code |
EH9 3AZ |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL25995 |
Illumina HiSeq 4000 (Heligmosomoides polygyrus bakeri; Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA512244 |
| SRA |
SRP174905 |
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