|
| Status |
Public on Jun 04, 2009 |
| Title |
Gene expression patterns under conditions of oxidative stress in Geobacter uraniumreducens |
| Platform organism |
Geotalea uraniireducens Rf4 |
| Sample organism |
Geotalea uraniireducens |
| Experiment type |
Expression profiling by array
|
| Summary |
Previous analysis of gene transcript levels of Geobacter species in groundwater during in situ
bioremediation of a uranium-contaminated aquifer detected expression of genes encoding
superoxide dismutase (sodA) and cytochrome d ubiquinol oxidase (cydA), proteins known to be involved in the response to oxidative stress in other microorganisms. In order to further elucidate gene expression patterns that could be attributed to oxygen
exposure, G. uraniumreducens was grown with acetate
as the electron donor and fumarate as the electron acceptor in the presence of oxygen and compared to non-oxygen treated cultures.
|
| |
|
| Overall design |
Three biological replicates were hybridized in duplicate. Experimental (oxygen treated) was labeled with cy5, control (no oxygen) was labeled with cy3.
|
| |
|
| Contributor(s) |
Mouser PJ, Holmes DE, Perpetua LA, Lovley DR |
| Citation(s) |
19129865 |
| |
| Submission date |
Aug 14, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Derek Lovley |
| Organization name |
University of Massachusetts Amherst
|
| Department |
Microbiology
|
| Lab |
D. R. Lovley
|
| Street address |
University of Massachusetts at Amherst, Dept. of Microbiology, Morrill IV north room 202
|
| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01003 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6566 |
Geobacter uraniumreducens Rf4 antisense-detecting array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA113015 |
Less...
More...