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| Status |
Public on May 24, 2023 |
| Title |
Transcriptomes change differently in different cancer cells upon SYK stable knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) to find out the difference between SYK stable knockdown and negative control group in each cancer cell line, and find the transcriptomes change upon SYK knockdown.
Methods: mRNA profiles of cell lines were generated using Illumina PE150, then GSEA was used for analyzing the association between SYK knockdown and the mesenchymal or epithelial signature.
Results: The result showed epithelial signatures were up-regulated, while mesenchymal signatures were down-regulated upon SYK knockdown in RNA-seq data.
Conclusions: Our study systematically examined the cellular transcriptomes affected by SYK knockdown, with biologic replicates, generated by RNA-seq technology. SYK knockdown results in the transcriptional change of multiple epithelial and mesenchymal markers, leading to mesenchymal state switching.
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| Overall design |
mRNA profiles of SYK stable knockdown and corresponding negative control generated from 3 cancer cell lines, individually.
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| Contributor(s) |
Geng M, Ding J |
| Citation(s) |
37183231 |
| |
| Submission date |
Jan 31, 2019 |
| Last update date |
May 24, 2023 |
| Contact name |
mengdi dai |
| E-mail(s) |
[email protected]
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| Organization name |
SIMM
|
| Street address |
555 Zuchongzhi Rd, Bldg. 2, RM.409 shanghai, P.R. China
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| City |
Shanghai |
| State/province |
China |
| ZIP/Postal code |
201203 |
| Country |
China |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA518088 |
| SRA |
SRP183068 |
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