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| Status |
Public on Jun 28, 2019 |
| Title |
Progressive dosage compensation during Drosophila embryogenesis is reflected by gene arrangement on the X chromosome [RNA-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In D. melanogaster males, X chromosome monosomy is compensated by chromosome-wide transcription activation. We found that complete dosage compensation during embryogenesis takes surprisingly long. Although the activating Dosage Compensation Complex (DCC) associates with the chromosome and acetylates histone H4 early, many genes are not compensated. Acetylation levels on gene bodies continue to increase for several hours after gastrulation in parallel with progressive compensation. Constitutive genes are compensated earlier than developmental genes. Remarkably, later compensation correlates with longer distances to DCC binding sites. This time-space relationship suggests that DCC action on target genes requires maturation of the active chromosome compartment.
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| Overall design |
RNA-seq in 8 embryonic stages in total 54 single embryos.
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| |
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| Contributor(s) |
Prayitno K, Schauer T, Becker PB |
| Citation(s) |
31286660 |
| |
| Submission date |
Feb 26, 2019 |
| Last update date |
Sep 27, 2019 |
| Contact name |
Tamas Schauer |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Zentrum München
|
| Department |
Institute of Epigenetics and Stem Cells
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| Street address |
Feodor-Lynen-Straße 21
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19951 |
Illumina HiSeq 1500 (Drosophila melanogaster) |
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| Samples (54)
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| This SubSeries is part of SuperSeries: |
| GSE127177 |
Progressive dosage compensation during Drosophila embryogenesis is reflected by gene arrangement on the X chromosome |
|
| Relations |
| BioProject |
PRJNA524294 |
| SRA |
SRP186866 |
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