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Series GSE127988 Query DataSets for GSE127988
Status Public on Oct 19, 2020
Title Gene expression of the A375 melanoma cell line treated with RAF inhibitors Vemurafenib and time-course with EGF stimulation during Vemurafenib RAF inhibitor treatment alone or with the MEK inhibitor Cobimetinib
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We treated for 24 hours the BRAF-V600E melanoma cell line A375 with 7 doses of the RAF inhibitor Vemurafenib and, in a second experimental desing, we treated for 24 hours the BRAF-V600E melanoma cell line A375 with Vemurafenib (1 uM) alone or in combination with the MEK inhibitor Cobimetinib (1 uM) and subsequently stimulated with EGF in a time-course of 7 time points for up to 8 hours (0, 0.5, 1, 2, 3, 4, 8 hours).
 
Overall design We measured mRNA levels of the A375 cell line treated for 24 hours with 7 doses of the RAF inhibitor Vemurafenib in the range 0.001-10 uM and, in a second experimental design, mRNA levels of the A375 cell line treated for 24 hours with the RAF inhibitor Vemurafenib (1 uM) alone or in combination with the MEK inhibitor Cobimetinib (1 uM) and subsequently stimulated with EGF (100 ng/mL) in a time-course up to 8 hours
 
Contributor(s) Gerosa L
Citation(s) 33113355
NIH grant(s)
Grant ID Grant title Affiliation Name
U54 CA225088 Systems Pharmacology of Therapeutic and Adverse Responses to ImmuneCheckpoint and Small Molecule Drugs HARVARD UNIVERSITY (MEDICAL SCHOOL) PETER Karl SORGER
Submission date Mar 07, 2019
Last update date Jan 18, 2021
Contact name Luca Gerosa
Organization name Harvard Medical School
Department Laboratory of Systems Pharmacology
Lab Peter Sorger Lab
Street address 200 Longwood Avenue Warren Alpert Bldg, Apt2
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (96)
GSM3659666 A375_Time=24_h_Vemurafenib=10_uM (S1)
GSM3659667 A375_Time=24_h_Vemurafenib=10_uM (S2)
GSM3659668 A375_Time=24_h_Vemurafenib=1_uM (S3)
Relations
BioProject PRJNA525999
SRA SRP187843

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Supplementary file Size Download File type/resource
GSE127988_RPM_Gerosa_et_al.csv.gz 13.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record
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