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| Status |
Public on Dec 21, 2019 |
| Title |
TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge [single-cell RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TREM2 is a microglial-specific gene implicated in late-onset Alzheimer's Disease (AD). Recent studies have identified that Trem2-deficient mice exhibit transcriptional changes in microglial gene expression that minimize the upregulation of lipid metabolism and lysosomal genes in AD disease models. We find that chronic phagocytic challenge from demyelination generates similiarly attenuated expression of lysosomal and lipid metabolism genes in microglia isolated from Trem2 knockout mouse brain.
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| Overall design |
Trem2 wildtype, heterozyogus knockout, and homozygous knockout mice were fed a control or 0.2% cuprizone diet for 5 weeks or 12 weeks to generate acute or chronic demyelination, respectively. Single-cell microglial gene expression was assessed using the Chromium Single Cell 3' Reagent Kit with v2 Chemistry (10X Genomics).
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| Contributor(s) |
Nugent AA, Lianoglou S, Sandmann T, Monroe KM, Di Paolo G |
| Citation(s) |
31902528 |
| |
| Submission date |
May 02, 2019 |
| Last update date |
Mar 22, 2020 |
| Contact name |
Thomas Sandmann |
| E-mail(s) |
[email protected], [email protected]
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| Organization name |
Denali Therapeutics
|
| Street address |
161 Oyster Point Blvd
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| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM3744996 |
Pooled microglia cells from two Trem2 +/+ animals after 12 weeks on control diet |
| GSM3744997 |
Pooled microglia cells from two Trem2 +/+ animals after 12 weeks on cuprizone diet |
| GSM3744998 |
Pooled microglia cells from two Trem2 +/- animals after 12 weeks on cuprizone diet |
| GSM3744999 |
Pooled microglia cells from two Trem2 -/- animals after 12 weeks on cuprizone diet |
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| This SubSeries is part of SuperSeries: |
| GSE130627 |
TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge |
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| Relations |
| BioProject |
PRJNA540830 |
| SRA |
SRP194567 |
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