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| Status |
Public on Jan 31, 2020 |
| Title |
RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal dendritic cell (DC) subsets, cDC1 and cDC2, as well as MHCII+CD64+ F4/80hi and MHCII+CD64+ CD11bhi cells. Splenic DCs and red pulp macrophages serve as reference populations for a macrophage-like or DC-like phenotype. By sorting YFP+/YFP- F4/80hi or YFP+/YFP- CD11bhi cells from Clec9a-Cre Rosa-YFP mice we aim to reveal transcriptional differences between YFP-labelled and unlabelled cells. We showed that F4/80hi cells resemble macrophages on a transcriptional level, despite their DC origin, and that renal CD11bhi cells are a transcriptionally unique subset. However, we were not able to reveal differences between YFP+ and YFP- populations.
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| |
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| Overall design |
RNAsequencing of murine dendritic cell and macrophage populations from kidney and spleen
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| |
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| Contributor(s) |
Rambichler S, Salei N, Schraml BU |
| Citation(s) |
31932472 |
| |
| Submission date |
May 24, 2019 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Barbara Schraml |
| E-mail(s) |
[email protected]
|
| Organization name |
LMU Munich
|
| Street address |
Großhaderner Strasse 9
|
| City |
Planegg |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (33)
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| Relations |
| BioProject |
PRJNA544681 |
| SRA |
SRP199438 |
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