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| Status |
Public on Apr 13, 2020 |
| Title |
eXcision Repair-sequencing (XR-seq) to map UV induced damage in U2OS cells and in U2OS cells in which CSA or UVSSA genes were knocked out |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used eXcision Repair-sequencing (XR-seq) to map UV induced damage in U2OS cells and in U2OS cells in which CSA or UVSSA genes were knocked out. Complementation of UVSSA knockout with WT or mutant proteins shows UVSSA is a core component of human transcription coupled repair.
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| Overall design |
We performed CPD XR-seq in U2OS WT, UVSSA-KO and CSA-KO, UVSSA-KO+GFP-UVSSA WT, UVSSA-KO+GFP-UVVSA-CIR, UVSSA-KO+GFP-UVVSA-TIR cells treated with UV irradiation (20J/m2).
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| Contributor(s) |
Golan Berman H, Heilbrun E, Luijsterburg MS, Adar S |
| Citation(s) |
32355176 |
| |
| Submission date |
Jun 17, 2019 |
| Last update date |
Jul 14, 2020 |
| Contact name |
Sheera Adar |
| Organization name |
Hebrew University of Jerusalem
|
| Department |
Department of Microbiology and Molecular Genetics
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| Lab |
Sheera Adar
|
| Street address |
Botnar Building Room 001, Faculty of Medicine, Hebrew University, Ein Kerem
|
| City |
Jerusalem |
| State/province |
Israel |
| ZIP/Postal code |
91120 |
| Country |
Israel |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA549258 |
| SRA |
SRP201609 |
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