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| Status |
Public on Aug 11, 2020 |
| Title |
Identification of ALPPL2 as a novel specific and functional surface marker for naïve pluripotency |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report ALPPL2 as a prominently specific and functional surface marker for naïve pluripotency. By performing RNA-seq analysis of TJ-1# and iCRISPR nESCs and pESCs, APG+ cells at different time points during naïve reprogramming, we validate ALPPL2’s specificity for naïve pluripotent state at transcriptional level. Deep analysis of the transcriptome differences between Wild Type (WT), ALPPL2 knockdown (AKD) cells via RNA-seq, Wild Type (WT), ALPPL2 knockout (AKO) and IGFBP1 knockout (IKO) individual colonies in detail via Smart-seq, and performing RNA immunoprecipitation (RIP) using IGF2BP1 antibody in nESCs followed by RNA-seq or qPCR analysis, we identify that ALPPL2 is essential for both establishment and maintenance of naïve pluripotency via interacting with IGF2BP1 to stabilize TFCP2L1 and STAT3 mRNA level.
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| Overall design |
Examination of mRNA profiles of WT, ALPPL2 KO, ALPPL2 KD, IGF2BP1 KO and IGF2BP1 enrichment by deep sequencing.
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| Contributor(s) |
Bi Y, Zhang Y, Wang Y |
| Citation(s) |
32187559 |
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| Submission date |
Jun 20, 2019 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Bi Yan |
| E-mail(s) |
[email protected]
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| Phone |
18916058932
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| Organization name |
Tongji university
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| Street address |
Siping Road 1239
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (2) |
| GPL20795 |
HiSeq X Ten (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (74)
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| Relations |
| BioProject |
PRJNA656543 |
| SRA |
SRP277076 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133097_RAW.tar |
25.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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