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| Status |
Public on Oct 22, 2019 |
| Title |
Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
A key step in microRNA (miRNA) biogenesis is the processing of a primary precursor RNA by the microprocessor into a precursor miRNA (pre-miRNA) intermediate. In plants, little is known about the processes that act on pre-miRNAs to influence miRNA biogenesis. Here, we performed 3’ RACE-seq to profile pre-miRNA 3’ ends in Arabidopsis. 3’ end heterogeneity was prevalent and the three microprocessor components promoted 3’ end precision. Extensive cytidylation and uridylation of precise and imprecise pre-miRNA 3’ ends were uncovered. The nucleotidyl transferase HESO1 uridylated pre-miRNAs in vitro and was responsible for most pre-miRNA uridylation in vivo. HESO1, NTP6 and NTP7 contribute to pre-miRNA cytidylation. Tailing of pre-miRNAs tended to restore trimmed pre-miRNAs to intact lengths to promote further processing. In addition, HESO1-mediated uridylation led to the degradation of some imprecisely processed pre-miRNAs. Thus, we uncovered widespread cytidylation and uridylation of pre-miRNAs and demonstrated diverse functions of pre-miRNA tailing in plants.
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| |
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| Overall design |
3' RACE-seq in Arabidopsis
|
| |
|
| Contributor(s) |
Song J |
| Citation(s) |
31792392 |
| |
| Submission date |
Jul 11, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Jianbo Song |
| E-mail(s) |
[email protected]
|
| Phone |
18579059335
|
| Organization name |
Jiang Xi Agricultural University
|
| Street address |
Department of Biochemistry and Molecular Biology, College of Science, Jiang Xi Agricultural University, Nanchang 330045, China
|
| City |
Nanchang |
| State/province |
Jiang Xi |
| ZIP/Postal code |
330045 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL21179 |
Illumina HiSeq 3000 (Arabidopsis thaliana) |
|
| Samples (63)
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|
| Relations |
| BioProject |
PRJNA554003 |
| SRA |
SRP214189 |
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