Expression profiling by high throughput sequencing
Summary
We developed a new single cell sequencing method to simultaneously identify RNA TSS and TES at single-cell level.
Overall design
Single DRG neurons, oocytes, hESC, HEk293T, ARPE, mESC, hESC_drived_organoid were sequenced by scRCAT-seq, a single cell transcriptomic protocol for identifying the transcription start sites and the transcription end sites. For comparison, Smart-seq2 and Iso-seq were also used to construct the libraries. scRCAT_seq_ERCC* samples are solely composed of the ERCC control RNAs. '100' and '1000' in the sample titles represent dilution. 0.1ul of 1:100 or 1:1000 dilution of ERCC was used to construct the library.
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