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| Status |
Public on Nov 07, 2008 |
| Title |
STOX1 overexpression in choriocarcinoma cells mimicks transcriptional alterations observed in preeclamptic placentas |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Background
Mutations in STOX1 were proposed as causal for predisposing to preeclampsia, a hypertensive disorder originating from placental defects, affecting up to 10% of human pregnancies. However, after the first study published in 2005 three other groups have dismissed the polymorphism described in the first paper as a causal mutation.
Methodology and Principal Findings
In the present study, we have developed a choriocarcinoma cell line overexpressing STOX1. This overexpression results in the transcriptional modification of 12.5% of the genes, some of them being direct targets as shown by chromatin immunoprecipitation. STOX1 overexpression correlates strongly and specifically with transcriptomic alterations in preeclamptic placentas (r=0.30, p=9.10-7). Numerous known key modulators of preeclampsia (such as Endoglin, Syncytin, human chrionic gonadotrophin-hCG-, and Glial Cell Missing Homolog -GCM1-) were modified in these transformed choriocarcinoma cells.
Conclusions
Our results contribute to reconcile contradictory data about the involvement of STOX1 in preeclampsia. In addition, they strongly suggest that anomalies in STOX1 expression are associated with the onset of preeclampsia, thus indicating that this gene should be the target of future studies. Furthermore, our cellular model could constitute an invaluable resource for studying specific aspects of this human disease.
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| Overall design |
Microarrays expression
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| Contributor(s) |
Rigourd V, Chauvet C, Chelbi ST, Rebourcet R, Mondon F, Letourneur F, Mignot T, Barbaux S, Vaiman D |
| Citation(s) |
19079545, 31098302 |
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| Submission date |
Nov 05, 2008 |
| Last update date |
May 21, 2019 |
| Contact name |
Daniel Vaiman |
| E-mail(s) |
[email protected]
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| Phone |
00 33 1 44412301
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| Fax |
00 33 1 44412302
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| Organization name |
INSERM
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| Department |
Genetics and Development
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| Lab |
GEPP
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| Street address |
24 rue du Faubourg St Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (2) |
| GSM339718 |
STOX1 overexpression in choriocarcinoma cells |
| GSM339719 |
Control transfection in choriocarcinoma cells |
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| Relations |
| BioProject |
PRJNA110151 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13475_RAW.tar |
10.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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