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| Status |
Public on Aug 13, 2019 |
| Title |
The conserved 3’ UTR-derived small RNA NarS counteracts the risk of toxic nitric oxide accumulation during nitrate respiration |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In pursuit of a biological role of Salmonella 3' UTR derived sRNA NarS, we sought to determine potential target mRNAs of NarS under anaerobic conditions. To this end, we compared gene expression in NarS-deficient and NarS overexpression (from plasmid pPL-NarS) strains following a 30-minute anaerobic shock by RNA-seq.
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| Overall design |
Total RNA of Salmonella NarS-deficient strain (ΔnarK ΔNarS) carrying either empty plasmid pJV300 or NarS-overexpressed plasmid pPL-NarS were compared.
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| Web link |
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz1168/5682904
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| Contributor(s) |
Wang C |
| Citation(s) |
31863581 |
| |
| Submission date |
Aug 12, 2019 |
| Last update date |
Dec 22, 2019 |
| Contact name |
Chuan Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
Fudan University
|
| Street address |
131 # Dong An Road
|
| City |
Shanghai |
| ZIP/Postal code |
200033 |
| Country |
China |
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| Platforms (1) |
| GPL27045 |
Illumina HiSeq 4000 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA559909 |
| SRA |
SRP218165 |
