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Series GSE13638 Query DataSets for GSE13638
Status Public on Feb 05, 2009
Title Ago2 or Dicer knockdown effects on mRNA levels in HeLa
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Analysis of mRNA changes in HeLa cells following Ago2 or Dicer depletion. Dicer, a cytoplasmic RNase III, generates the mature form of small RNAs including microRNA. Ago2 is a component of an effector complex of microRNA.

Keywords: gene expression array-based (RNA / in situ oligonucleotide)
 
Overall design siRNA against Ago2 or Dicer were trasnfected into HeLa cells. siRNA against GFP was used as a control. Biologically duplicated total RNAs were prepared from HeLa cells, 24 hrs and 48 hrs after siRNA transfection.
 
Contributor(s) Han J, Kim Y, Kim VN
Citation(s) 19135890
Submission date Nov 18, 2008
Last update date Jul 26, 2018
Contact name Jinju Han
Organization name Seoul National University
Lab Bd. 504, Rm 501
Street address 599 Gwanangno, Gwanak-gu
City Seoul
ZIP/Postal code 151-742
Country South Korea
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM343388 HeLa_siGFP_24_rep1
GSM343389 HeLa_siGFP_24_rep2
GSM343390 HeLa_siAgo2_24_rep1
This SubSeries is part of SuperSeries:
GSE13640 Effect of miRNA biogenesis factors on mRNA levels
Relations
BioProject PRJNA113875

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13638_RAW.tar 49.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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