|
| Status |
Public on Sep 17, 2019 |
| Title |
Identification of a unique subtype of lung squamous cell carcinoma defined by SOX2 and a neural differentiation factor BRN2 [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Lineage-specific transcriptional regulators control differentiation states not only during normal development but also during cancer evolution. By investigating super-enhancer landscape of lung squamous cell carcinoma (LUSC), we identified a unique ‘neural’ subtype defined by Sox2 and a neural lineage factor Brn2. Robust protein-protein interaction and genomic co-occupancy of these factors indicated their transcriptional cooperation in this ‘neural’ LUSC in contrast to the cooperation of Sox2 and p63 in the classical LUSC. Introduction of p63 expression in the “neural’ LUSC invoked the classical LUSC lineage accompanied by Brn2 downregulation and increased activities of ErbB/Akt and MAPK-ERK pathways. Collectively, our data demonstrate a unique LUSC lineage featured by Sox2 cooperation with Brn2 instead of p63, for which distinct therapeutic approaches may be warranted.
|
| |
|
| Overall design |
Transcriptomic profiles in control and DNp63-overexpressed LK2 cells
|
| |
|
| Contributor(s) |
Sato T, Watanabe H, Yoo S |
| Citation(s) |
31551362 |
| |
| Submission date |
Sep 16, 2019 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Hideo Watanabe |
| E-mail(s) |
[email protected]
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Department of Medicine, Division of Pulmonary, Critical Care and Sleep Medicine
|
| Lab |
Hideo Watanabe
|
| Street address |
One Gustave L. Levy Place
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
| GSM4079790 |
GFP-overexpressed LK2 cells_1 [CTRL_1] |
| GSM4079791 |
GFP-overexpressed LK2 cells_2 [CTRL_2] |
| GSM4079792 |
inducible DNp63-expressing LK2 cells without doxycycline_replicate1 [CTRL_3] |
| GSM4079793 |
inducible DNp63-expressing LK2 cells without doxycycline_replicate2 [CTRL_4] |
| GSM4079794 |
DNp63-overexpressed LK2 cells_1 [OE_1] |
| GSM4079795 |
DNp63-overexpressed LK2 cells_2 [OE_2] |
| GSM4079796 |
inducible DNp63-expressing LK2 cells with doxycycline_replicate1 [OE_3] |
| GSM4079797 |
inducible DNp63-expressing LK2 cells with doxycycline_replicate2 [OE_4] |
|
| This SubSeries is part of SuperSeries: |
| GSE137461 |
Identification of a unique subtype of lung squamous cell carcinoma defined by SOX2 and a neural differentiation factor BRN2 |
|
| Relations |
| BioProject |
PRJNA565678 |
| SRA |
SRP221712 |
Less...
More...