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| Status |
Public on Aug 21, 2020 |
| Title |
Gene expression profiling of MDA_MB_231 cell line in response to treatment with Dexamethasone and SH4-54 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine how the transcription factors GR and STAT3 cooperate to regulate gene expression, the MDA-MB-231 breast cancer cell line was treated with dexamethasone (1µM for 24 hours) and the STAT3 inhibitor SH4-54 (8µM for 24 hours) alone and in combination. A multivariate linear model with a GR:STAT3 interaction term was used to identify genes that are differentially expressed when both TFs are fully active in the nucleus.
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| Overall design |
MDA-MB-231 cells were plated in 6-well plates at ~300,000 cells in 4ml of media per well and grown for 24 hours. Three replicates wells were treated for 24 hours with 1µM of Dexamethasone (DEX), 8µM SH4-54, no treatment, or a combination of DEX and SH4-54. The cells were lysed using Qiagen RLT buffer and 1% BME.
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| Web link |
https://cancerres.aacrjournals.org/content/early/2020/08/19/0008-5472.CAN-20-1379
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| Contributor(s) |
Conway M, Varley KE |
| Citation(s) |
32816914 |
| |
| Submission date |
Sep 16, 2019 |
| Last update date |
Nov 05, 2020 |
| Contact name |
Katherine E Varley |
| Organization name |
University of Utah
|
| Department |
Oncological Sciences
|
| Street address |
2000 Cir of Hope Dr.
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA565792 |
| SRA |
SRP221806 |
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