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Status |
Public on Oct 04, 2019 |
Title |
Propranolol exhibits activity against hemangiomas independent of beta blockade |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Propranolol is a widely used beta blocker that consists of a racemic mixture of R and S stereoisomers. Only the S stereoisomer has significant activity against the beta-adrenergic receptor. A fortuitous clinical observation was made in an infant who received propranolol for cardiac disease, and regression of a hemangioma of infancy was noted. order to gain further insights in the mechanisms of action of R-propranolol in vivo, we subjected vehicle- and R-propranolol-treated tumors to RNAseq analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that choline metabolism was the pathway most affected by R-propranolol treatment. Consistent with this, the most highly regulated gene by R-propranolol treatment was Betaine-Homocysteine Methyl Transferase (Bhmt). Other genes that are involved in tumor suppressive activities such as Early Growth Response 1 (Egr1) and AP-1 subunit, which are both transcription factors implicated in tumor suppression. Egr1 regulates important tumor suppressors such as PTEN and p53, and upregulates tumor necrosis factor α (TNF-α). Fos, which has been known to be rearranged and expressed frequently in epithelioid hemangioma, was also upregulated in R-propranolol-treated samples. The genes identified in the RNAseq analysis pose as interesting targets for further investigations.
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Overall design |
Analysis of transcriptome regulation in R-propranolol treated tumors
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Contributor(s) |
Sasaki M, North P, Elsey J, Bubley J, Rao S, Jung Y, Wu S, Zou M, Pollack BP, Kumar J, Singh H, Arbiser JL |
Citation(s) |
31701018 |
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Submission date |
Oct 03, 2019 |
Last update date |
Jan 03, 2020 |
Contact name |
Jack L Arbiser |
E-mail(s) |
[email protected]
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Phone |
404-727-5063
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Organization name |
Emory School of Medicine
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Department |
Dermatology
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Lab |
Arbiser
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Street address |
101 Woodruff Circle WMB 5309
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City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (9)
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Relations |
BioProject |
PRJNA575740 |
Supplementary file |
Size |
Download |
File type/resource |
GSE138403_Gene_Counts_unfiltered.txt.gz |
348.9 Kb |
(ftp)(http) |
TXT |
Raw data are available in SRA |
Processed data are available on Series record |
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