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| Status |
Public on Jan 26, 2020 |
| Title |
Single cell analysis reveals human cytomegalovirus drives latently infected cells towards an anergic-like monocyte state |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human cytomegalovirus (HCMV) causes a lifelong infection through establishment of latency. Although reactivation from latency can cause life-threatening disease, our molecular understanding of HCMV latency is incomplete. Here we use single cell RNA-seq analysis to characterize latency in monocytes and hematopoietic stem and progenitor cells (HSPCs). In monocytes, we identify host cell surface markers that enable enrichment of latent cells harboring higher viral transcript levels, which can reactivate more efficiently, and are characterized by an intrinsic reduced immune response that is critical for viral gene expression. Significantly, in latent HSPCs, viral transcripts could be detected only in monocyte progenitors and were also associated with reduced immune-response. Overall, our work demonstrates that regardless of the developmental stage in which HCMV infects, HCMV drives hematopoietic cells towards a weaker immune-responsive monocyte state and that this anergic-like state is crucial for the virus ability to express its transcripts and to eventually reactivate.
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| Overall design |
MARS-Seq bulk protocol was used for monocytes-derived macrophages, monocytes FACS-sorted according to the levels of DC74, and monocytes with and without treatment by ruxolitinib, all infected with HCMV.
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| Contributor(s) |
Shnayder M, Nachshon A, Bernstein B, Fein N, Rozman B, Poole E, Lavi M, Avdic S, Blyth E, Gottlieb D, Abendroth A, Slobedman B, Sinclair J, Stern-Ginossar N, Schwartz M |
| Citation(s) |
31967545 |
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| Submission date |
Oct 15, 2019 |
| Last update date |
Jan 27, 2020 |
| Contact name |
Noam Stern-Ginossar |
| E-mail(s) |
[email protected]
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| Organization name |
Weizmann Institute
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| Department |
Molecular Genetics
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| Lab |
Noam Stern-Ginossar
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| Street address |
234 herzel
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA577574 |
| SRA |
SRP225665 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138838_cd74_high_vs_low.txt.gz |
297.5 Kb |
(ftp)(http) |
TXT |
| GSE138838_macrophages.txt.gz |
98.7 Kb |
(ftp)(http) |
TXT |
| GSE138838_ruxo_kinetics.txt.gz |
390.2 Kb |
(ftp)(http) |
TXT |
| GSE138838_ruxo_plus_vs_minus.txt.gz |
400.4 Kb |
(ftp)(http) |
TXT |
| GSE138838_sample_barcodes.txt.gz |
340 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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