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| Status |
Public on Oct 01, 2020 |
| Title |
Transcriptomic data of established human AT/RT cell lines BT-12. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The multi-subunit ATP-dependent chromatin remodeling complex BAF ('mammalian SWI/SNF complex') regulates DNA accessibility during cell fate transitions and functions as a context-dependent tumor suppressor. SMARCB1, as a one of the component of BAF complex, was first found to be biallelically inactivated in ~98% of all malignant rhabdoid tumors (MRT), aggressive pediatric tumors that can occur cranially or extracranially. To examine the mechanisms through which inactivation of SMARCB1 drives malignant rhabdoid tumorigenesis, we conducted RNAseq studies in BT-12 cells reconstituted with either WT or M4 mutant (an in-frame deletion (185-193D) was identified from tumor patient) SMARCB1. These results indicate that loss of SMARCB1 promotes cell proliferation but also impairs cell adhesion and migration, consistent with the hypothesis that SMARCB1 exerts its tumor suppressive function predominantly by inhibiting proliferation.
We applied the RNA-Seq to provide the transcriptomic profiling of BT-12 cell lines without or with stable expression of SMARCB1.
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| Overall design |
RNA-Seq of BT-12 cell lines have 3 replicates each.
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| |
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| Contributor(s) |
Zhou H, Wang Y |
| Citation(s) |
33024572 |
|
https://doi.org/10.1038/s41421-020-00196-4
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| |
| Submission date |
Oct 22, 2019 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Filippo Giancotti |
| E-mail(s) |
[email protected]
|
| Organization name |
UT MD Anderson Cancer Center
|
| Department |
Cancer Biology
|
| Lab |
Dr. Giancotti Lab
|
| Street address |
1881 East Road
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA578927 |
| SRA |
SRP226637 |
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