|
| Status |
Public on Nov 19, 2009 |
| Title |
Transcriptome of mature Arabidopsis thaliana crown galls |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
This study describes physiological changes, morphological adaptations and the regulation of pathogen defense responses in Arabidopsis crown galls. Crown gall development was induced on intact plants under most natural conditions with Agrobacterium tumefaciens. Differential gene expression and the metabolite pattern was determined by comparing crown galls with mock-inoculated inflorescence stalk segments of the same age.
|
| |
|
| Overall design |
The bases of Arabidopsis thaliana (WS-2) inflorescence stalks were wounded and immediately inoculated with Agrobacterium tumefaciens, strain C58, or mock-inoculated. Plants were cultivated for another 35 days under short day conditions (8 h illumination, 16 h darkness). Gene expression values of four independent experiments of treated material (C58 35dpi 1 to 4) were compared with four non-treated samples of the same age (reference 35dpi 1 to 4). Differential gene expression was analyzed by applying the LIMMA package (Linear Models for Microarray Data; Smyth, G.K. (2004) Applic. Genet. Mol. Biol. 3, Article 3; http://www.bepress.com/sagmb/vol3/iss1/art3/).
|
| |
|
| Contributor(s) |
Deeken R, Engelmann J, Mueller T |
| Citation(s) |
17172353, 17827272 |
| |
| Submission date |
Dec 12, 2008 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Rosalia Deeken |
| E-mail(s) |
[email protected]
|
| Phone |
+49 931 8886121
|
| Fax |
+49 931 8886158
|
| Organization name |
University of Wuerzburg, Julius-von-Sachs-Institute
|
| Department |
Botany I; Plant Molecular Physiology & Biophysics
|
| Lab |
Prof. Hedrich
|
| Street address |
Julius-von-Sachs-Platz 2
|
| City |
Wuerzburg |
| ZIP/Postal code |
97072 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE14106 |
Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens |
|
| Relations |
| BioProject |
PRJNA114605 |
Less...
More...