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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 06, 2020 |
| Title |
Genome-wide CRISPR screen of rituximab induced cell depletion |
| Organisms |
Homo sapiens; unidentified plasmid |
| Experiment type |
Other
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| Summary |
We screened the genome of OCI-Ly-7, a Diffuse Large B-cell Lymphoma cell line, for genes mediating the response to rituximab treatment. We utilized the genome-wide library GeCKO v2 (A+B) from Feng Zhang. (Addgene#1000000049)
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| Overall design |
In addition to mock treated sample, we subjected cells to two different rituximab conditions. RTX-SEC (short exposure to CDC conditions, SEC, followed by non-CDC conditions for a total of 14 days) and RTX-REC (repeated exposure to CDC conditions, REC, maintained for 21 days). The rationale was to expose cells in the RTX-SEC group shortly to CDC conditions followed by non-CDC conditions, whereas cells in the RTX-REC group were repeatedly subjected to conditions supporting CDC
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| Contributor(s) |
Thomsen EA, Mikkelsen JG |
| Citation(s) |
32585766 |
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| Submission date |
Oct 25, 2019 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Søren Riis Paludan |
| E-mail(s) |
[email protected]
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| Organization name |
Aarhus University
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| Department |
Biomedicin
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| Lab |
Søren Riis Paludan
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| Street address |
Høegh-Guldbergs Gade 10
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| City |
Aarhus |
| ZIP/Postal code |
8000 Aarhus C |
| Country |
Denmark |
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| Platforms (2) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA579532 |
| SRA |
SRP226990 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139385_RTX_screen_mageck.count.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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