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| Status |
Public on Nov 12, 2019 |
| Title |
Integrated transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We employed high-fat diet (HFD)-induced prediabetes and HFD/streptozotocin (STZ)-induced type 2 diabetes mouse models that develop PN, which we compared to control mice maintained on standard diet (SD) and to HFD and HFD/STZ mice that were subject to SD dietary reversal (DR) half-way through the study. We performed gene expression profiling on sciatic nerves from SD, HFD, HFD-STZ, HFD-DR, and HFD-STZ-DR animal cohorts. Nerve transcriptomic profiles were integrated using complex correlation analyses, and biological meaning was inferred from known lipid-gene interactions in the literature
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| Overall design |
mRNA profiles of sciatic nerve (SCN) tissue was isolated from 16-week and 24-week old C57BL/6J mice that were under . Our prediabetic model consisted of BL/6J mice fed a HFD (60% kcal fat) from 5 weeks of age. Our T2D diabetic model consisted of 12-week old HFD mice injected with low dose streptozotocin (STZ; 75mg/kg followed by a 50mg/kg dose 3 days later (i.p. injection)). At 16 weeks, subgroups of HFD and HFD-STZ mice were switched to a control diet (13% kcal fat) for 8 weeks to mimic dietary reversal. RNA-seq of SCN was performed on SCN tissue at the 16 week and 24 week timepoints.
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| Contributor(s) |
Hur J, Guo K, Feldman EL |
| Citation(s) |
31822493 |
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| Submission date |
Nov 11, 2019 |
| Last update date |
Feb 10, 2020 |
| Contact name |
Junguk Hur |
| E-mail(s) |
[email protected]
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| Organization name |
University of North Dakota
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| Department |
Biomedical Sciences
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| Street address |
1301 N. Columbia Rd. Stop 9037
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| City |
Grand Forks |
| State/province |
ND |
| ZIP/Postal code |
58202-9037 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (53)
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| Relations |
| BioProject |
PRJNA588782 |
| SRA |
SRP229489 |
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