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| Status |
Public on Jun 01, 2020 |
| Title |
Genome-wide maps of O-GlcNAc proteins in human breast cancer cells by next generation sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We applied the chemical reporter-based metabolic labeling method to acquire O-GlcNAc modified proteins chromatin loci. Human breast cancer cell line MCF-7, as well as the genotoxic stress (Adriamycin) adapted cells MCF-7/ADR, were fed with 1 mM GalNAz. Metabolic labeled O-GlcNAz chromatin were crosslinked, sonicated and enriched by bioorthogonal chemistry. Then, the genomic DNA fragments bounded by O-GlcNAc mark were de-crosslinked, and constructed into libraries following by next-generation sequencing (Chemoselective O-GlcNAc chromatin sequencing, COGC-seq). To verify the robustness of this chemical reporter-based metabolic labeling method, we compared the results in MCF-7 and MCF-7/ADR cells with classical lectin succinylated wheat germ agglutinin (sWGA) ChIP-seq strategy. We also analyzed gene expression MCF-7 and MCF-7/ADR cells by RNA-seq.
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| Overall design |
Examination O-GlcNAc protein bound chromatin loci in human breast cancer cells MCF-7 and MCF-7/ADR cells. Gene expression profiling of MCF-7 and MCF-7/ADR cells were performed by RNA-seq.
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| Contributor(s) |
Liu Y, Zhang N, Cao Y, Dou T, Zhang J |
| Citation missing |
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| Submission date |
Dec 09, 2019 |
| Last update date |
Sep 06, 2020 |
| Contact name |
bo yu liu |
| E-mail(s) |
[email protected]
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| Phone |
15842607600
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| Organization name |
Dalian University of Technology
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| Street address |
Dagong Road 2
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| City |
panjin |
| State/province |
liaoning |
| ZIP/Postal code |
124000 |
| Country |
China |
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| Platforms (1) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA594402 |
| SRA |
SRP235291 |
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