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| Status |
Public on Aug 23, 2020 |
| Title |
RNA-seq analysis after Ssrp1 knockout or Usp7 depletion in embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Endogenous retrovirus MERVL is specifically expressed in a minority of embryonic stem cells. To determine the restrain mechanism of MERVL, we knocked out Ssrp1 and analyzed the effect on the expression of transposable elements and coding genes. Ssrp1 further interacts with ubiquitin specific protease Usp7. We knocked down usp7 and analyzed the effect on the expression of MERVL. It turns out the deletion of ssrp1 or usp7 would lead to upregulation of MERVL. This study extends our understandings of the machanism by novel factors regulates MERVL.
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| Overall design |
We knocked out Ssrp1 in E14 by CRISPR/Cas9 System. And we knocked down Usp7 in E14 by shRNA. We performed RNA-seq analysis in order to exam the transcriptome after Ssrp1 KO or Usp7 knockdown.
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| |
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| Contributor(s) |
Lu X, Chen F, Zhang W |
| Citation(s) |
32894293 |
| |
| Submission date |
Dec 10, 2019 |
| Last update date |
Oct 26, 2020 |
| Contact name |
Weiyu Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
18902012072
|
| Organization name |
Nankai University
|
| Street address |
No.38 Tongyan Road, Jinnan District
|
| City |
Tainjin |
| ZIP/Postal code |
300350 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA594729 |
| SRA |
SRP235595 |
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