|
| Status |
Public on Dec 30, 2020 |
| Title |
Non-CG methylation is more potent than CG methylation in moss genome regulation [Ppatens RNA-Seq] |
| Organism |
Physcomitrium patens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cytosine methylation is commonly targeted to symmetrical CG sites, as well as to non-CGs, such as the symmetrical-CHG and asymmetric-CHH sites in plants (H= A, C or T). Thus far, depletion of CG methylation in plants was associated with ample transcriptional activation of transposons. Here, we profiled transcription in various context specific methylation mutants in the early-diverged plant, Physcomitrella patens. We discovered that specific elimination of CG methylation is fully complemented by non-CG methylation but not vice versa. Between the symmetrically-methylated sites, CHG methylation silenced transposons stronger than CG methylation did. Finally, non-CG methylation revealed as crucial for the silencing of CG depleted transposons. Our results suggest that non-CG methylation evolved to silence transposons due to functional limitations and/or rapid mutability of methylated-CGs.
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| |
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| Overall design |
mRNA-sequencing of P. patens wild type and single and double DNMT mutant samples (drm1drm2, met, cmt, dnmt3b, met/dnmt3b, cmt/dnmt3b)
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| |
|
| Contributor(s) |
Domb K, Katz A, Yaari R, Kaizler E, Hoang Nguyen V, Vu Thuy Hong U, Griess O, Harris KD, Gitin Heskiau K, Ohad N, Zemach A |
| Citation missing |
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| |
| Submission date |
Dec 15, 2019 |
| Last update date |
Dec 30, 2020 |
| Contact name |
Katherine Domb |
| E-mail(s) |
[email protected]
|
| Organization name |
Tel Aviv University
|
| Department |
School of Plant Sciences and Food Security
|
| Lab |
Assaf Zemach
|
| Street address |
Haim Levanon st.
|
| City |
Tel-Aviv |
| ZIP/Postal code |
6997801 |
| Country |
Israel |
| |
|
| Platforms (2) |
| GPL17793 |
Illumina HiSeq 2500 (Physcomitrella patens) |
| GPL27916 |
Illumina NextSeq 500 (Physcomitrella patens) |
|
| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE142054 |
Non-CG methylation is more potent than CG methylation in moss genome regulation |
|
| Relations |
| BioProject |
PRJNA595744 |
| SRA |
SRP237661 |
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