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| Status |
Public on Feb 06, 2020 |
| Title |
Cripto shapes macrophage plasticity and restricts EndMT in injured and diseased skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The membrane protein Cripto plays a key role in shaping macrophage plasticity in skeletal muscle during regeneration and disease. Cripto acts as an extrinsic modulator of macrophage plasticity and is required for the proper expansion/maintenance of the CD206+ anti-inflammatory macrophage population. Nevertheless, Cripto deletion does not change the gene expression profile of F4/80+/Ly6CLow macrophages suggesting that Cripto was dispensable to induce/maintain the Ly6CLow phenotype.
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| Overall design |
RNAseq analysis: total RNA from FACS isolated macrophages (F4/80+/Ly6CLow) from WT and Tg:LysMCre::Cripto-/- mice was extracted using a protocol combining QIAzol Lysis Reagent and RNAeasy minikit columns (Qiagen) following the manufacturer’s instructions. RNAseq services were provided by the CNIC Genomics Unit, including quality control tests of total RNA using Agilent Bioanalyzer and Nanodrop. spectrophotometry. cDNA library preparation and amplification were performed from 200 ng total RNA using NEBNext Ultra RNA Library Prep Kit for Illumina. RNAseq analysis was performed with 3 samples per condition, using Illumina Hiseq 2500.
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| Contributor(s) |
Perdiguero E, Minchiotti G, Iavarone F |
| Citation(s) |
32107853 |
| |
| Submission date |
Dec 16, 2019 |
| Last update date |
Jul 07, 2021 |
| Contact name |
Eusebio Perdiguero |
| E-mail(s) |
[email protected]
|
| Organization name |
UPF
|
| Department |
Department of Medicine and Life Sciences
|
| Lab |
Cell Biology
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
|
| GSM4219519 |
LysMCre-Cripto-deficient macrophages Rep 1 |
| GSM4219520 |
LysMCre-Cripto-deficient macrophages Rep 2 |
| GSM4219521 |
LysMCre-Cripto-deficient macrophages Rep 3 |
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| Relations |
| BioProject |
PRJNA595859 |
| SRA |
SRP237750 |
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