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| Status |
Public on Jan 03, 2009 |
| Title |
Gene expression signatures for 17α-ethinylestradiol and ZM 189,154, singly and combined, in fathead minnow testis |
| Organism |
Pimephales promelas |
| Experiment type |
Expression profiling by array
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| Summary |
Aquatic organisms are continuously exposed to complex mixtures of chemicals, many of which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order to analyze the effects and mechanisms of action of estrogen/anti-estrogen mixtures, we exposed male fathead minnows (Pimephales promelas) for 48 hours via the water to 2, 5, 10, and 50 ng 17α- ethinylestradiol (EE2)/L, 100 ng ZM 189,154/L (a potent antiestrogen known to block activity of all estrogen receptors) or mixtures of 5 or 50 ng EE2/L with 100 ng ZM 189,154/L. We analyzed gene expression changes in the gonad, as well as hormone and vitellogenin plasma levels. Steroidogenesis was down-regulated by EE2 as reflected by the reduced plasma levels of testosterone in the exposed fish and down-regulation of genes in the steroidogenic pathway. Microarray analysis of testis of fathead minnows treated with 5 ng EE2/L or with the mixture of 5 ng EE2/L and 100 ng ZM 189,154/L indicated that some of the genes whose expression was changed by EE2 were blocked by ZM 189,154, while others were either not blocked or enhanced by the mixture, generating two distinct expression patterns. Gene ontology and pathway analysis programs were used to determine categories of genes for each expression pattern. Our results suggest that response to estrogens occurs via multiple mechanisms, including canonical binding to soluble estrogen receptors, membrane estrogen receptors, and other mechanisms that are not blocked by pure antiestrogens.
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| Overall design |
Fathead minnows exposed to 5, 10, or 50 ng/L ethinylestradiol, 100 ng/L ZM189,154, or a mixture of 5 or 50 ng/L ethinylestradiol and 100 ng/L ZM189,154. There are four replicates per treatment. We used a reference design.
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| Contributor(s) |
Reyero Vinas NG, Kroll KJ, Liu L, Orlando E, Watanabe KH, Sepulveda MS, Villeneuve DL, Perkins EJ, Ankley GT, Denslow ND |
| Citation(s) |
19594897 |
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| Submission date |
Dec 30, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Natalia Vinas |
| E-mail(s) |
[email protected], [email protected]
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| Phone |
6016343764
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| Organization name |
Mississippi State University
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| Street address |
3909 Halls Ferry Rd
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| City |
Vicksburg |
| State/province |
MS |
| ZIP/Postal code |
39180 |
| Country |
USA |
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| Platforms (2) |
| GPL6516 |
US EPA Ecoarray FHM Pimephales promelas 10K 2000 gene (pilot) array |
| GPL7282 |
EcoArray Fathead minnow 44K v1.0 |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA111155 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14235_RAW.tar |
310.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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