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| Status |
Public on Apr 02, 2021 |
| Title |
Transcriptomic analysis by RNA-seq unveils distinct gene expression profile of U397 cells depleted of NSPs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Characterization of transcriptomic changes associated with genetic ablation of three neutrophil serine proteases (CTSG, ELANE, PRTN3). U937 cells depleted of CTSG, ELANE, and PRTN3 simultaneously (ΔNSPs) were generated using the CRISPR-Cas9 system and sgRNAs. RNA-Seq analysis revealed transcriptomic changes between control cells and ΔNSPs, with 170 and 220 genes up- or down-regulated respectively.
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| Overall design |
RNA-Seq analysis of three control lines of U937 cells and three independent clones of ΔNSPs U937 cells
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| Contributor(s) |
Cheung P, Schaffert S, Chang SE, Mellins ED, Khatri P, Utz PJ, Kuo AJ |
| Citation(s) |
34017121 |
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| Submission date |
Dec 27, 2019 |
| Last update date |
May 23, 2021 |
| Contact name |
Purvesh Khatri |
| Organization name |
Stanford University
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| Department |
BMIR
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| Street address |
279 Campus Dr. Beckman Center B235A
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA597899 |
| SRA |
SRP238918 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142662_counts_raw.csv.gz |
236.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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