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| Status |
Public on Jan 31, 2021 |
| Title |
Integrative analysis of reference epigenomes in polyploid crop Brassica napus L. [WGBS] |
| Organism |
Brassica napus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We establish global maps of regulatory elements and chromatin states and their dynamics, for both the whole and subgenomes of four tissue types (young leaf, flower bud, silique, and root) of two B. napus lines. Approximately 52 % of the genome was annotated with different epigenomic signals. We also uncover a new bivalent chromatin state in B. napus and suggest its key roles in regulating tissue-specific gene expression. Furthermore, we observe that different types of duplicated genes have differential patterns of histone modifications, DNA methylation, and selection pressures. Together, we provide valuable epigenetic data resources of allopolyploid B. napus and reveal the central role of epigenomic information in understanding transcriptional regulation in polyploid plants.
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| Overall design |
Here, we generated genome-wide profiling of five histone modifications and RNA polymerase II occupancy using an enhanced chromatin immunoprecipitation (eChIP) approach. By integrating DNA methylation, and transcriptome datasets, we constructed comprehensive epigenome landscapes across various tissues in B. napus.
WGBS: Total genomic DNA was extract from tissue with DNeasy plant mini kit (QIAGEN, 69104), library construction using EZ DNA Methylation-Gold kit (ZYMO, D5005), sequence was generated on Illumina HiSeq X Ten sequencer.
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| Contributor(s) |
Zhang Q, Guan P, Zhao L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 08, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Guan Peng Peng |
| E-mail(s) |
[email protected]
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| Organization name |
Huazhong Agricultural University
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| Department |
college of informatics
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| Street address |
shizishan street
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| City |
WuHan |
| State/province |
HuBei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE143287 |
Integrative analysis of reference epigenomes in polyploid crop Brassica napus L. |
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| Relations |
| BioProject |
PRJNA599592 |
| SRA |
SRP240296 |
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