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| Status |
Public on May 07, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of transcriptomes in ARP-1 cells treated with DMSO and carfilzomib (Cfz) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goals of NGS-derived retinal transcriptome profiling (RNA-seq) is to identify the signaling pathways in multiple myeloma (MM) cells affected by Cfz treatment. We treated ARP-1 MM cells with DMSO or 6nM Cfz for 16 hours (each group has 3 replicates) and extracted the total RNA using RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to BGI genomics for RNA-seq followed by data analysis.
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| |
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| Overall design |
Examination of gene-profiling data in MM cells repsonse to control or Cfz treatment.
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| Contributor(s) |
Wang Q |
| Citation(s) |
32582913 |
| |
| Submission date |
Jan 09, 2020 |
| Last update date |
Aug 06, 2020 |
| Contact name |
Qiang Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
HOUSTON METHODIST RESEARCH INSTITUTE
|
| Street address |
6550 FANNIN STREET
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA600307 |
| SRA |
SRP241044 |
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